All of the extreme measures to "protect you" against the imaginary virus ironically destroy your immune system. Masks, isolation, social distancing, being inside (not getting sunlight and fresh air) are absolutely detrimental to one's health.
So why is he investing billions of dollars and devoting his life to AI development amongst other Dystopian nightmare society things.
Have you stocked up on food yet? With the "Second Wave" (Worldwide) and election chaos (USA) coming, things are going to get a bit crazy for a little while. In addition to this, the Cult/Cabal has been hard at work to create a massive food shortage worldwide.
"Don't be scared, just be prepared for the worst."
"Don't be scared, just be prepared for the worst."
COVID-19 Vaccines:
All Transfection Methods will use (Luciferase Gene)
3 Methods of Transfection
1) Lipid Based Transfection
2) Calcium Phosphate
3) Electroporation (Use of Electricity)
1st Transfection Method: Lipid based
Transfection Method: Lipid (Luciferase Gene)
Enhanced in vivo bioluminescence imaging using liposomal luciferin delivery system
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2815139/
A Potent Branched-Tail Lipid Nanoparticle Enables Multiplexed mRNA Delivery and Gene Editing In Vivo
https://pubs.acs.org/doi/10.1021/acs.nanolett.0c00596
Quantum Dots & Nanoparticles
Functionalized lipid-like nanoparticles for in vivo mRNA delivery and base editing
https://advances.sciencemag.org/content/6/34/eabc2315
Lipid Nanoparticles for Gene Delivery
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5006671/
Lipid-mRNA Nanoparticle Designed to Enhance Intracellular Delivery Mediated by Shock Waves
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6602409/
2nd Transfection Method: Calcium Phosphate
Transfection Method: Calcium Phosphate (Luciferase Gene)
Non-Invasive In Vivo Imaging of Calcium Signaling in Mice
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1991622/
Transfection of Luciferase with Delivery System of Nanoparticles
Calcium phosphate nanoparticles-based systems for siRNA delivery
https://academic.oup.com/rb/article/3/3/187/1752885
Quantum Dots & Nanoparticles
Preliminary in vivo magnetofection data using magnetic calcium phosphate nanoparticles immobilizing DNA and iron oxide nanocrystals
https://www.sciencedirect.com/science/article/pii/S2352340918304116
Biodegradable Calcium Phosphate Nanoparticle with Lipid Coating for Systemic siRNA Delivery
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2833237/
3rd Transfection Method: Electroporation
Transfection Method: Electroporation (Luciferase Gene)
Electroporation as a method for high-level nonviral gene transfer to the lung
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5576138/
In vivo electroporation and ubiquitin promoter--a protocol for sustained gene expression in the lung
https://pubmed.ncbi.nlm.nih.gov/16685743/
A Critical Review of Electroporation as A Plasmid Delivery System in Mouse Skeletal Muscle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6600476/
Visualization of in vivo electroporation mediated transgene expression in experimental tumors by optical and magnetic resonance imaging
https://www.nature.com/articles/gt200955
Electroporation of Mammalian Cells with a Firefly Luciferase Expression Plasmid: Kinetics of Transient Expression Differ Markedly among Cell Types
https://www.liebertpub.com/doi/10.1089/dna.1.1988.7.557
Quantum Dots & Nanoparticles
Gold Nanoparticles Enhanced Electroporation for Mammalian Cell Transfection
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4696415/
Electroporation for nanomedicine: a review
https://pubs.rsc.org/en/content/articlelanding/2017/tb/c7tb00038c#!divAbstract
Electroporation of a nanoparticle-associated DNA vaccine induces higher inflammation and immunity compared to its delivery with microneedle patches in pigs
https://www.sciencedirect.com/science/article/pii/S0168365919303840
All Transfection Methods will use (Luciferase Gene)
3 Methods of Transfection
1) Lipid Based Transfection
2) Calcium Phosphate
3) Electroporation (Use of Electricity)
1st Transfection Method: Lipid based
Transfection Method: Lipid (Luciferase Gene)
Enhanced in vivo bioluminescence imaging using liposomal luciferin delivery system
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2815139/
A Potent Branched-Tail Lipid Nanoparticle Enables Multiplexed mRNA Delivery and Gene Editing In Vivo
https://pubs.acs.org/doi/10.1021/acs.nanolett.0c00596
Quantum Dots & Nanoparticles
Functionalized lipid-like nanoparticles for in vivo mRNA delivery and base editing
https://advances.sciencemag.org/content/6/34/eabc2315
Lipid Nanoparticles for Gene Delivery
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5006671/
Lipid-mRNA Nanoparticle Designed to Enhance Intracellular Delivery Mediated by Shock Waves
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6602409/
2nd Transfection Method: Calcium Phosphate
Transfection Method: Calcium Phosphate (Luciferase Gene)
Non-Invasive In Vivo Imaging of Calcium Signaling in Mice
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1991622/
Transfection of Luciferase with Delivery System of Nanoparticles
Calcium phosphate nanoparticles-based systems for siRNA delivery
https://academic.oup.com/rb/article/3/3/187/1752885
Quantum Dots & Nanoparticles
Preliminary in vivo magnetofection data using magnetic calcium phosphate nanoparticles immobilizing DNA and iron oxide nanocrystals
https://www.sciencedirect.com/science/article/pii/S2352340918304116
Biodegradable Calcium Phosphate Nanoparticle with Lipid Coating for Systemic siRNA Delivery
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2833237/
3rd Transfection Method: Electroporation
Transfection Method: Electroporation (Luciferase Gene)
Electroporation as a method for high-level nonviral gene transfer to the lung
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5576138/
In vivo electroporation and ubiquitin promoter--a protocol for sustained gene expression in the lung
https://pubmed.ncbi.nlm.nih.gov/16685743/
A Critical Review of Electroporation as A Plasmid Delivery System in Mouse Skeletal Muscle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6600476/
Visualization of in vivo electroporation mediated transgene expression in experimental tumors by optical and magnetic resonance imaging
https://www.nature.com/articles/gt200955
Electroporation of Mammalian Cells with a Firefly Luciferase Expression Plasmid: Kinetics of Transient Expression Differ Markedly among Cell Types
https://www.liebertpub.com/doi/10.1089/dna.1.1988.7.557
Quantum Dots & Nanoparticles
Gold Nanoparticles Enhanced Electroporation for Mammalian Cell Transfection
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4696415/
Electroporation for nanomedicine: a review
https://pubs.rsc.org/en/content/articlelanding/2017/tb/c7tb00038c#!divAbstract
Electroporation of a nanoparticle-associated DNA vaccine induces higher inflammation and immunity compared to its delivery with microneedle patches in pigs
https://www.sciencedirect.com/science/article/pii/S0168365919303840
PubMed Central (PMC)
Enhanced in vivo bioluminescence imaging using liposomal luciferin delivery system
To provide a continuous and prolonged delivery of the substrate D-luciferin for bioluminescence imaging in vivo, luciferin was encapsulated into liposomes using either the pH-gradient or acetate-gradient method. Under optimum loading conditions, 0.17 ...