🎙للذي يسال كيف افعل تقرير 🔝
🔝سنلخص في موضوعنا هذا بسرعه نوع بكتيري واحد وكلها نفس الخطوات فقط في اختلاف بالفحوصات التي بتشتغلها حسب نوع البكتيريا🦠

🔬Microbial Examination Report:-

1⃣day 1
🔸Wet preparation :-
We seen Pus cells and Bactria
🔸Culture on B.A , M.A and Choc.A

2⃣Day 2
🔹Examination and report Culture :-
if growth or non growth
🔻on M.A non growth
🔺on B.A >

🔺large colony
🔺regular colony
🔺same if hemolysis (beta hemolysis ) clear zone around colony
🔺Same do not hemolysis with gray - white colony
🔺on Choc.A
Appear large colony
Regular colony
White colony with out hemolysis
🔖ملاحضة
كتبت في قراءة التزريع في طبق choc.A
With out hemolysis
للتأكيد على ان التحلل يتم البحث عن في طبق B.A فقط
🔹Gram stain :-
▪️نفرض انها Gram positive cocci in groups
🔸Subculture:- on M.S.A
Or other plate
🔸do catalase :-
🔖Positive result⬆️bubbles
🔖negative result non⬆️bubbles
لنفرض هنا انه إجابي catalase
🔹do coagulase :-
Positive clot
نفرض انه سلبي coagulase -ve
3⃣day 3
🔎Examination and report subculture :- (M.S.A) or other
🔺large colony
🔺yellow colony
🔺raised colony
🔺circular colony
🔺smooth colony
🔹Do antibiotic sensitivity test:-

4⃣Day 4
🔎read result of antibiotic sensitivity test like Novobiocin
🔖sensitive
🔖resistant
نفرض انه resistant
⬆️result report ➡️S.suprophyticus

#منشور_بسيط
وضحنا فيه طريقة كتابه التقرير وحبنا مثال عن التقرير حتى وصلنا للنتيجة النهائية للبكتيريا
وكل تقرير يوجد فيه اختلاف بسيط بنو الفحوصات bichemicals ونوع الميديا المزرع فيها لكن الطريقة مافيها اي اختلاف
د.عبدالله احمد
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#Diognosis_of_Microbiology
#منتدى_الطب_المخبري

🔝hus
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@MicroMLS1

🔝An Outbreak of Hemolytic Uremic Syndrome Associated
with Antibiotic Treatment of Hospital Inpatients for
Dysentery
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@MicroMLS1

risk_of_hemolytic_uremic_syndrome_after_antibiotic_safdar.pdf

🔝Treatment options for HUS secondary to Escherichia
coli O157:H7
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@MicroMLS1

📘Harrison's Infectious Diseases
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@MicroMLS1

📘Harrison's Infectious Diseases
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@MicroMLS1

🔝2017 Harrison's Infectious Diseases, 3rd Edition
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@MicroMLS1

🔝2017 Harrison's Infectious Diseases, 3rd Edition
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@MicroMLS1

الية مقاومة البكتيريا للمضادات الحيويه

🔝V.cholerae
🔝Oxidase +ve
🔝Tcbs (SF)
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@MicroMLS1

🔝V.cholerae
🔝Tcbs (SF)
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@MicroMLS1

🔝V.cholerae
🔝Oxidase +ve
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@MicroMLS1

🔝Indole -ve
🔝KIA y/y
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@MicroMLS1

🔝Pseudomonas aeruginosa
🕳oxidase positive
🧪KIA R/R
🧪Citrate positive
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@LabMed2016

🕳String test vebrio
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@MicroMLS1

🔝Str.pneumoniae
🕳Optochin sensetive
🕳 alpha hemolysis
🕳bile solubility test +ve
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@MicroMLS1

🔝للذي يسال عن فحص string test
string test
*⃣to differentiate vibrio cholerae postive from Aermonas spp negative both are isolate from diarrhed stool..

*⃣to differentiate vibrio cholerae postive from other vibrio spp negative..
🔴procedure ..التحضير
🔹put drop of 0.5/. Bile salt on clean slid..
🔹emulsify an asolated colony of bacterium using an inoculating loop
🔹keep on rubbring the loop vigorously for2-3mins until it appears viscous
🔹gently pull the loop up wards from the slid
🔻result→format of thread like mucoid string indicates postive result
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@MicroMLS1

🕳String test for the identification of Vibrio cholerae


🔝Vibrio cholerae is a gram-negative bacilli bacteria with a “comma” shaped body and a flagella for the movement. 

🦠V. cholerae and V. mimicus are the only Vibrio spp.
that do not require salt for growth.
🕳 The string test may be performed on a glass microscope slide or plastic petri dish by suspending 18 to 24 hour growth from Heart Infusion Agar (HIA) or other non-inhibitory medium in a drop of 0.5% aqueous solution of sodium deoxycholate.

🔝Principle of String test🔝

🎙If the result is positive, the bacterial cells will be lysed by the sodium deoxycholate or Sodium taurocholate, the suspension will lose turbidity, and DNA will be released from the lysed cells causing the mixture to become viscous.
A mucoid “string” is formed when an inoculating loop is drawn slowly away from the suspension.

🔝Uses of String Test🔝
String test is used t o separate Vibrio spp. from Aeromonas spp. and P. shigelloides.

🦠String test is used to differentiate Vibrio cholerae string test positive from other Vibrio spp string test negative
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