Forwarded from DeMa
COVID-19 Vaccines:
All Transfection Methods will use (Luciferase Gene)
3 Methods of Transfection
1) Lipid Based Transfection
2) Calcium Phosphate
3) Electroporation (Use of Electricity)
1st Transfection Method: Lipid based
Transfection Method: Lipid (Luciferase Gene)
Enhanced in vivo bioluminescence imaging using liposomal luciferin delivery system
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2815139/
A Potent Branched-Tail Lipid Nanoparticle Enables Multiplexed mRNA Delivery and Gene Editing In Vivo
https://pubs.acs.org/doi/10.1021/acs.nanolett.0c00596
Quantum Dots & Nanoparticles
Functionalized lipid-like nanoparticles for in vivo mRNA delivery and base editing
https://advances.sciencemag.org/content/6/34/eabc2315
Lipid Nanoparticles for Gene Delivery
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5006671/
Lipid-mRNA Nanoparticle Designed to Enhance Intracellular Delivery Mediated by Shock Waves
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6602409/
2nd Transfection Method: Calcium Phosphate
Transfection Method: Calcium Phosphate (Luciferase Gene)
Non-Invasive In Vivo Imaging of Calcium Signaling in Mice
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1991622/
Transfection of Luciferase with Delivery System of Nanoparticles
Calcium phosphate nanoparticles-based systems for siRNA delivery
https://academic.oup.com/rb/article/3/3/187/1752885
Quantum Dots & Nanoparticles
Preliminary in vivo magnetofection data using magnetic calcium phosphate nanoparticles immobilizing DNA and iron oxide nanocrystals
https://www.sciencedirect.com/science/article/pii/S2352340918304116
Biodegradable Calcium Phosphate Nanoparticle with Lipid Coating for Systemic siRNA Delivery
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2833237/
3rd Transfection Method: Electroporation
Transfection Method: Electroporation (Luciferase Gene)
Electroporation as a method for high-level nonviral gene transfer to the lung
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5576138/
In vivo electroporation and ubiquitin promoter--a protocol for sustained gene expression in the lung
https://pubmed.ncbi.nlm.nih.gov/16685743/
A Critical Review of Electroporation as A Plasmid Delivery System in Mouse Skeletal Muscle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6600476/
Visualization of in vivo electroporation mediated transgene expression in experimental tumors by optical and magnetic resonance imaging
https://www.nature.com/articles/gt200955
Electroporation of Mammalian Cells with a Firefly Luciferase Expression Plasmid: Kinetics of Transient Expression Differ Markedly among Cell Types
https://www.liebertpub.com/doi/10.1089/dna.1.1988.7.557
Quantum Dots & Nanoparticles
Gold Nanoparticles Enhanced Electroporation for Mammalian Cell Transfection
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4696415/
Electroporation for nanomedicine: a review
https://pubs.rsc.org/en/content/articlelanding/2017/tb/c7tb00038c#!divAbstract
Electroporation of a nanoparticle-associated DNA vaccine induces higher inflammation and immunity compared to its delivery with microneedle patches in pigs
https://www.sciencedirect.com/science/article/pii/S0168365919303840
All Transfection Methods will use (Luciferase Gene)
3 Methods of Transfection
1) Lipid Based Transfection
2) Calcium Phosphate
3) Electroporation (Use of Electricity)
1st Transfection Method: Lipid based
Transfection Method: Lipid (Luciferase Gene)
Enhanced in vivo bioluminescence imaging using liposomal luciferin delivery system
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2815139/
A Potent Branched-Tail Lipid Nanoparticle Enables Multiplexed mRNA Delivery and Gene Editing In Vivo
https://pubs.acs.org/doi/10.1021/acs.nanolett.0c00596
Quantum Dots & Nanoparticles
Functionalized lipid-like nanoparticles for in vivo mRNA delivery and base editing
https://advances.sciencemag.org/content/6/34/eabc2315
Lipid Nanoparticles for Gene Delivery
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5006671/
Lipid-mRNA Nanoparticle Designed to Enhance Intracellular Delivery Mediated by Shock Waves
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6602409/
2nd Transfection Method: Calcium Phosphate
Transfection Method: Calcium Phosphate (Luciferase Gene)
Non-Invasive In Vivo Imaging of Calcium Signaling in Mice
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1991622/
Transfection of Luciferase with Delivery System of Nanoparticles
Calcium phosphate nanoparticles-based systems for siRNA delivery
https://academic.oup.com/rb/article/3/3/187/1752885
Quantum Dots & Nanoparticles
Preliminary in vivo magnetofection data using magnetic calcium phosphate nanoparticles immobilizing DNA and iron oxide nanocrystals
https://www.sciencedirect.com/science/article/pii/S2352340918304116
Biodegradable Calcium Phosphate Nanoparticle with Lipid Coating for Systemic siRNA Delivery
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2833237/
3rd Transfection Method: Electroporation
Transfection Method: Electroporation (Luciferase Gene)
Electroporation as a method for high-level nonviral gene transfer to the lung
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5576138/
In vivo electroporation and ubiquitin promoter--a protocol for sustained gene expression in the lung
https://pubmed.ncbi.nlm.nih.gov/16685743/
A Critical Review of Electroporation as A Plasmid Delivery System in Mouse Skeletal Muscle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6600476/
Visualization of in vivo electroporation mediated transgene expression in experimental tumors by optical and magnetic resonance imaging
https://www.nature.com/articles/gt200955
Electroporation of Mammalian Cells with a Firefly Luciferase Expression Plasmid: Kinetics of Transient Expression Differ Markedly among Cell Types
https://www.liebertpub.com/doi/10.1089/dna.1.1988.7.557
Quantum Dots & Nanoparticles
Gold Nanoparticles Enhanced Electroporation for Mammalian Cell Transfection
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4696415/
Electroporation for nanomedicine: a review
https://pubs.rsc.org/en/content/articlelanding/2017/tb/c7tb00038c#!divAbstract
Electroporation of a nanoparticle-associated DNA vaccine induces higher inflammation and immunity compared to its delivery with microneedle patches in pigs
https://www.sciencedirect.com/science/article/pii/S0168365919303840
PubMed Central (PMC)
Enhanced in vivo bioluminescence imaging using liposomal luciferin delivery system
To provide a continuous and prolonged delivery of the substrate D-luciferin for bioluminescence imaging in vivo, luciferin was encapsulated into liposomes using either the pH-gradient or acetate-gradient method. Under optimum loading conditions, 0.17 ...
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Episode 11 of @thewayfwrd podcast featuring Dr. Larry Palevsky is live! Amazing discussion on health, the way we learn, critical thinking, pharma-influence, vaccines, & adopting a child-like approach to learning.
This quote comes from Episode 11 of @thewayfwrd podcast w/ Dr Palevsky.
How do you see the world?
Have you done the work to address the biases, the preconceived notions, the blind acceptances, the trauma, the many things impressed upon you- both consciously & subconsciously? Are your perceptions derived from within you? Why do you feel that way when that happens? Why do you think that way? Why does my intuition not match what I’ve been told? Why do my eyes see something different than what I’ve been taught? Why do so many others have the same story? Why are they dismissed as well? Why have we accepted what we’ve accepted? Are these blind acceptances actually true?
Cover all of the eyes that are telling you how to see- what do you see?
How do you see the world?
Have you done the work to address the biases, the preconceived notions, the blind acceptances, the trauma, the many things impressed upon you- both consciously & subconsciously? Are your perceptions derived from within you? Why do you feel that way when that happens? Why do you think that way? Why does my intuition not match what I’ve been told? Why do my eyes see something different than what I’ve been taught? Why do so many others have the same story? Why are they dismissed as well? Why have we accepted what we’ve accepted? Are these blind acceptances actually true?
Cover all of the eyes that are telling you how to see- what do you see?
Forwarded from Ali Zeck (Ali Zeck)
Links:
Gates and investments in world health return :
https://fortune.com/2019/01/17/bill-gates-best-investment-vaccines-global-health/
He lied about his relationship with Epstein
http://techrights.org/2020/08/21/bill-gates-lies-re-jeffrey-epstein/
Software engineer arrested at G8tes’ home:
https://www.seattlepi.com/seattlenews/article/Gates-estate-staff-member-sentenced-for-child-6344956.php
Antitrust lawsuit:
https://corporatefinanceinstitute.com/resources/knowledge/strategy/microsoft-antitrust-case/
Paul Allen, former partner calls him ruthless schemer
https://www.independent.co.uk/news/people/news/gates-is-a-ruthless-schemer-says-his-microsoft-co-founder-2257843.htm
India:
https://m.economictimes.com/industry/healthcare/biotech/healthcare/controversial-vaccine-studies-why-is-bill-melinda-gates-foundation-under-fire-from-critics-in-india/articleshow/41280050.cms
Africa
https://theduran.com/un-forced-to-admit-gates-funded-vaccine-is-causing-polio-outbreak-in-africa-21st-century-wire/
Gates and investments in world health return :
https://fortune.com/2019/01/17/bill-gates-best-investment-vaccines-global-health/
He lied about his relationship with Epstein
http://techrights.org/2020/08/21/bill-gates-lies-re-jeffrey-epstein/
Software engineer arrested at G8tes’ home:
https://www.seattlepi.com/seattlenews/article/Gates-estate-staff-member-sentenced-for-child-6344956.php
Antitrust lawsuit:
https://corporatefinanceinstitute.com/resources/knowledge/strategy/microsoft-antitrust-case/
Paul Allen, former partner calls him ruthless schemer
https://www.independent.co.uk/news/people/news/gates-is-a-ruthless-schemer-says-his-microsoft-co-founder-2257843.htm
India:
https://m.economictimes.com/industry/healthcare/biotech/healthcare/controversial-vaccine-studies-why-is-bill-melinda-gates-foundation-under-fire-from-critics-in-india/articleshow/41280050.cms
Africa
https://theduran.com/un-forced-to-admit-gates-funded-vaccine-is-causing-polio-outbreak-in-africa-21st-century-wire/
Fortune
Bill Gates Says These Global Health Investments Were the Best He’s Ever Made. Here’s Why
Investing $10 billion has yielded about $200 billion in economic and social benefits, Gates says.
https://www.mdpi.com/2076-393X/8/4/676?s=03 brand new study 👀
MDPI
Multiple Vaccinations and the Enigma of Vaccine Injury
A growing number of vaccines are administered at the same time or in close succession, increasing the complexity of assessing vaccine safety. Individual vaccines are assumed to have no other effect than protection against the targeted pathogen, but vaccines…
another brilliant piece by former Medical Corps Officer & ‘97 West Point graduate Pam Long https://childrenshealthdefense.org/defender/military-flu-vaccine-mandate/
Children's Health Defense
Military’s Flu Vaccine Mandate Is Cash Cow for Big Pharma, But Wrong for Service Members
It’s time to stop forcing military members to take a vaccine that’s ineffective, increases health risks and violates medical ethics.